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| 产品名称 | Recombinant Mouse IL-13 (106AA) | 蛋白编号 | P20109 |
| 产品别称 | Interleukin-13; IL-13; T-Cell Activation Protein P600; Il13; Il-13 | ||
| 产品描述 | Recombinant Mouse Interleukin-13 is produced by our E.coli expression system and the target gene encoding Ser26-Phe131 is expressed. | ||
| 分子量 | Mol Mass:11.7 KDa; APMol Mass:9-14 KDa, reducing conditions | 剂型描述 | Lyophilized from a 0.2 μm filtered solution of 20mM Histidine-HCl, 8% Trehalose, 4% Mannitol, 50mM NaCl, 0.05% Tween 80, pH 6.0. |
| 内毒素 | < 1 EU/µg as determined by LAL test. | 纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| 生物活性 | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1.93 ng/ml. | ||
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | ||
| 保存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. | ||
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. | ||
| 产品背景 | Mouse interleukin 13 (mIL-13) is a pleiotropic cytokine produced by activated Th2 cells. IL-13 induces B cell proliferation and immunoglobin production. It contains a four helical bundle with two internal disulfide bonds. Mouse IL13 shares 58% sequence identity with human protein and exhibits cross-species activity. IL13 signals via receptor IL13R (type2, IL4R) and activates STAT-6. IL13 initially binds IL-13Rα1 with low affinity and triggers association of IL4Rα, generating a high affinity heterodimeric receptor IL13R and eliciting downstream signals. IL13 also binds IL-13Rα2 with high affinity, which plays a role in a negative feedback system of IL13 signaling. IL13 is an important mediator of allergic inflammation and disease. | ||
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Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
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Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1.93 ng/ml.