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| 产品名称 | Recombinant Human IL-1b | 蛋白编号 | P01584 |
| 产品别称 | Interleukin-1 beta; Catabolin; IL1F2; IL1B | ||
| 产品描述 | Recombinant Human Interleukin-1 Beta is produced by our E.coli expression system and the target gene encoding Ala117-Ser269 is expressed. | ||
| 分子量 | Mol Mass:17.5 KDa; APMol Mass:17 KDa, reducing conditions | 剂型描述 | Lyophilized from a 0.2 μm filtered solution of 20mM PB，150 mM NaCl，3% Trehalose，0.02% Tween 80，4% Mannitol，pH 7.4. |
| 内毒素 | < 0.01 EU/µg as determined by LAL test. | 纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| 生物活性 | Measured by its ability to induce NF-kB signaling in 293-IL1 Res cells. The ED50 for this effect is 20-100 pg/ml. (QC verified) | ||
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | ||
| 保存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. | ||
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. | ||
| 产品背景 | IL1B belongs to the IL-1 family. Interleukin 1 (IL-1) is a family of polypeptide cytokines consisting of two agonists, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2) encoded by two distinct genes and perform identical biological functions. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response. It is identified as endogenous pyrogens, and is reported to stimulate the release of prostaglandin and collagenase from synovial cells. | ||
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Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
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Measured by its ability to induce NF-kB signaling in 293-IL1 Res cells. The ED50 for this effect is 20-100 pg/ml. (QC verified)